Analysis of Polyoxazolines

Polyoxazolines are polymers which are used – chemically bound to an active pharmaceutical ingredient - for massive half-life elongation of pharmaceuticals in the bloodstream of animals and humans. Especially for long-term treatments this is very beneficial for patients since intervals between treatments can be significantly extended.

Our team has extensive experience analysing PEtOx and PMeOx using HPLC-MS/MS with low detection limits in biological matrix such as rat plasma for example.

For a 5 kDa PEtOx an LLOQ of about 2 ng/mL is achievable in rat plasma; for a 25 kDa PEtOx an LLOQ of about 40 ng/mL is achievable; for a 40 kDa PEtOx an LLOQ aofabout 20 ng/mL; and for a 500 kDa PEtOx an LLOQ of about 80 ng/mL.

Chromatograms are depicted for:
• 5 kDa PEtOX (Top down: Blank rat plasma; spiked rat plasma at 16 ng/mL; spiked rat plasma at 80 ng/mL)
• 40 kDa PEtOX (Top down: Blank rat plasma; spiked rat plasma at 16 ng/mL; spiked rat plasma at 80 ng/mL)
• 500 kDa PEtOX (Top down: Blank rat plasma; spiked rat plasma at 80 ng/mL)